Knowing more about our CAA TEST

INSTITUT KURZ

CAA TEST


 

KNOWING MORE ABOUT OUR CAA TEST


CAA TEST

CAA (Cellular Antioxidant Activity): The assay consists of measuring the antioxidant activity of a compound within human cells. It is a relevant method with respect to chemical tests for measuring antioxidant activity because it takes into account some cellular events such as absorption, metabolism and position of antioxidant compounds within cells. The assay involves the use of a probe that has the ability to oxidize itself into a fluorescent molecule; the antioxidant compounds inhibit the formation of free radicals and therefore the increase in fluorescence (Fig. 1).
The method can be applied to both food ingredients and nutritional supplements.

                                       
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Cell test

Figure 1: modified from Bender et al, Am J Biol Life Sci 2014; 2(3): 72-79. (A) Cells are treated with the selected compounds (AOx) and the dichlorofluorescein diacetate molecule, DCF-DA, which is absorbed by cells by passive diffusion and deacetylated by cell esterases to non-fluorescent DCFH. (B) DCFH is rapidly oxidized by free radicals, produced by 2,2'-Azobis [2-methylpropionamide] dihydrochloride, AAPH, a peroxyl radical initiator. Free radicals lead to the oxidation of DCFH to DCF which emits fluorescence proportional to the level of oxidation. Under these conditions, a decrease in the fluorescence signal compared to untreated cells indicates the antioxidant capacity of the tested compound, as antioxidants react with peroxyl radicals preventing the formation of fluorescent DCF dichlorofluorescein.


Institut Kurz

is one of the few laboratories able to carry out analysis to determine the antioxidant power of numerous ingredients using different human cell lines.
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